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Digesting vector MINV-IRES-mB7-2 by enzyme MuntI and BamHI to obtain IRES-mB7-2 gene fragment with ambly end and BamHI sticking end. 2MunI酶切载体MINV-IRES-mB7-2,末端钝化,然后BamHI再酶切,得到含钝末端和BamHI粘末端的IRES-mB7-2基因片段;